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. 2020 Apr 8;318(6):C1107–C1122. doi: 10.1152/ajpcell.00061.2020

Fig. 1.

Fig. 1.

Verification of c42-GAL4 activity in Malpighian tubules of female Drosophila melanogaster. A: control stain (w;+/+;c42-GAL4/+) yields Malpighian tubules of adult Drosophila melanogaster lacking enhanced green fluorescent protein (eGFP). B–I: eGFP expression in w;+/+;c42-GAL4/UAS-eGFP. B: stage 13 embryo;no distinct eGFP expression is visible. C: stage 17 embryo;first eGFP expression becomes apparent in Malpighian tubules and salivary glands. D–F: c42-GAL4 activity in 1st, 2nd, and 3rd larval instars, respectively. Additional eGFP expression is visible in salivary gland cells and in pericardial cells (asterisks). G: tubules isolated from 3rd instar larvae. H and I: adult Malpighian tubules. Arrowheads in G–I point to stellate cells that do not express eGFP.