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Quantitative PCR of act5C-GAL4-driven knockdown of Tsp2A. Expression of Tsp2A in the cross act5C-GAL4 × w1118 serves as control (100% Tsp2A expression). The RNAi lines VDRC_101081 and 11415-R2 induced, respectively, moderate and strong knockdown of Tsp2A. Female flies of Drosophila melanogaster were maintained at 25°C. Statistical significant difference was evaluated with the Student’s t test. Data are means ± SE of 3 biological replicates.
