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Effect of strong Tsp2A knockdown (UAS line 11415-R2) on the localization of Tsp2A and Dlg (A–C’), Ssk and Dlg (D–F’), and Mesh and Dlg (G–I’) in the main secretory segment of anterior Malpighian tubules of adult female Drosophila melanogaster. DNA revealed by propidium iodide staining identifies cell nuclei. Flies were reared at 27°C. An affinity purified antibody against Tsp2A was used.
