Fig. 2.

Expression pattern of intraflagellar transport 81 (IFT81) in the seminiferous tubules and its localization in male germ cells of wild-type mice. A: examination of IFT81 expression in testis by immunochemistry staining. IFT81 was expressed in all stages (I–XII) of seminiferous tubules. The protein was not present in the cells near the basement of the seminiferous tubules (white arrows). Strong signal was detected in spermatocytes and round spermatids (black arrows). B: immunofluorescence staining of IFT81 in seminiferous tubules of wild-type mice. IFT81 was present in the cytoplasm of spermatocytes (arrows in a) and round spermatids (dashed arrows in b). C: localization of IFT81 in the isolated germ cells of wild-type mice. a: IFT81 was present as cytoplasm in round spermatids. SP56, an acrosome marker, was double stained. b: The cells were double stained with a manchette marker (α-tubulin). IFT81 (arrowhead pointing to red) was not colocalized with α-tubulin. c: IFT81 was present in the developing tails (white arrows). Ab, antibody.