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. 2020 Jun 17;11:631. doi: 10.3389/fgene.2020.00631

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Copyright © 2020 Soubeyrand, Nikpay, Lau, Turner, Hoang, Alain and McPherson.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CARMAL is polyadenylated and stable. (A) CARMAL and PPIA levels were determined on cDNA prepared from HEK293T RNA in the presence of either random hexamer primer (poly(A) tail independent), oligodT anchor primer (poly(A) tail dependent) or a 1:1 mixture of both (standard condition), to which values are normalized. Results represent the mean of 3 biological replicates (±SD). (B) CARMAL (filled symbols; C) and TRIB1 (an unstable mRNA control; matching empty symbols; T) expression levels measured in cells treated with either Actinomycin D or vehicle DMSO) only. Values represent the ratio of the relative RNA abundance (relative to PPIA) in Actinomycin D treated cells over the vehicle treated cells. Data from three to five biological replicates are shown (±95% CI). *p < 0.05. Changes for TRIB1 were all highly significant (***p < 0.005).