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. 2020 Jun 17;11:631. doi: 10.3389/fgene.2020.00631

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Copyright © 2020 Soubeyrand, Nikpay, Lau, Turner, Hoang, Alain and McPherson.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CARMAL suppression by KRABMeCP2-dCas9 is associated with increased MFGE8 levels. HEK293T cells were transfected for 72 h with the indicated CRISPRi fusion constructs together with one of three distinct gRNA or a control gRNA (tracrRNA + linker only) expressing constructs. For each biological replicate, inhibition (vs trcrRNA control) values obtained using each gRNA were averaged. Values represent the average of three distinct biological replicates (±95% CI) *, <0.05 vs CRISPRi/trcrRNA only; ^§p < 0.05 vs normalized SRP14 values (one-way ANOVA).