FIGURE 7.

Splicing in WT and CARMAL deleted HEK293T differ. Wild-type (WT) or CARMAL deleted cells in 24 well plates were transfected for 24 h with variable amounts (μg) of either an intron-containing luciferase construct or an intronless control [CMV-LUC2CP/intron/ARE and CMV2-LU2CP/ARE (Addgene)], together with 1% of Renilla internal control. Four independent experiments were performed over a 2 week period. (A) Representative experiment is shown (average of two distinct DNA preparations ± SD). (B) Average ability to process intron (intron/intronless values) of the CARMAL deleted cells relative to the Wt controls, over four experiments (±SD). Values per dose (in ng per well) of transfected DNA are shown.