TABLE 2.

Gene set enrichment analysis of CARMAL KO cells implicates chromatin remodeling.

Gene set	Description	Size	Leading edge number	ES	NES	FDR
R-HSA-3214847	HATs acetylate histones	128	36	0.21176	2.4582	0.046996
R-HSA-2559582	Senescence-Associated Secretory Phenotype	96	46	0.23162	2.3856	0.054247
R-HSA-3214858	RMTs methylate histone arginines	68	31	0.27329	2.4868	0.063334
R-HSA-201722	Formation of the beta-catenin:TCF complex	79	29	0.23953	2.2332	0.076631
R-HSA-3214815	HDACs deacetylate histones	81	33	0.23395	2.285	0.080407
R-HSA-3214842	HDMs demethylate histones	42	29	0.30264	2.2356	0.087934
R-HSA-8878171	Transcriptional regulation by RUNX1	220	60	0.14351	2.0917	0.088852
R-HSA-73728	RNA Polymerase I Promoter Opening	51	24	0.28042	2.169	0.09081
R-HSA-3247509	Chromatin modifying enzymes	254	85	0.13944	2.1375	0.091122
R-HSA-4839726	Chromatin organization	254	85	0.13944	2.1375	0.091122

GSEA was used to interrogate a list of genes ranked according to the change in expression magnitude observed between the CARMAL KO and controls. The submitted list contained 29590 IDs of which 25339 IDs mapped to Entrez Gene IDs. Among these, 9818 IDs mapped to functional Reactome categories. Results shown include all hits with FDR <0.1. Gene Set: Reactome identifier; Description: Reactome synonym. Size, number of molecules in the identifier; Leading Edge number: number of genes populating the gene set that drive the enrichment signal; ES: enrichment score; NES: normalized enrichment score; FDR: false discovery rate to correct for multiple hypothesis testing. Analysis was performed via WebGestalt.