Table 2:
Comparison of semi nested mulitiplex PCR and qRT-PCR-HRM methods with the microscopy as gold standard in detecting asymptomatic malaria in Jalalabad City, Afghanistan
| Variable | Reference | Total | Validity | ||
|---|---|---|---|---|---|
| SNM-PCR | Microscopy | Sensitivity (95%Cl) | Specificity (95%Cl) | ||
| Positive | Negative | 100% (47.8 – 100) | 91.1% (86.1 – 94.7) | ||
| Positive | 5 | 17 | 22 | ||
| Negative | 0 | 274 | 274 | ||
| Total | 5 | 291 | 296 | ||
| Qrt-PCR-HRM | Positive | Negative | Sensitivity (95%Cl) | Specificity (95%Cl) | |
| Positive | 5 | 21 | 26 | 100% (47.8 – 100) | 92.7% (89.1 – 95.4) |
| Negative | 0 | 270 | 270 | ||
| Total | 5 | 291 | 296 | ||