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. 2020 May 18;13(5):916–922. doi: 10.14202/vetworld.2020.916-922

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Copyright: © Mengfan, et al.

Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Figure-3 — Phylogenetic analysis of different geographical strains of Ehrlichia canis based on amino acid sequences of gp36 protein. The amino acid sequences of gp36 protein of E. canis obtained in this study and available in GenBank were used to construct phylogenetic tree by the neighbor-joining method. Bootstrap values were calculated with 1000 replicates. Filled circle indicates E. canis strain XJ-2, XJ-6, XJ-21, and XJ-35 identified in this study. The GenBank accession numbers of 42 strains of E. canis were as follows: DJ (North Carolina), DQ146153.1; Florida, DQ146152.1; Cameroon 71, DQ146155.1; Sao Paulo, DQ146154.1; Louisiana, DQ146151.1; Demon, DQ085429.1; Oklahoma, DQ085428.1; Jake, DQ085427.1; Londrina, JX312080; 70C, KF233414.1; 56C, KF233413.1; 1055, KT363877.1; 533, KT363876.1; 36, KT363875.1; CM196, MF771085.1; CM180, MF771084.1; 222, KC479021.1; 171, KC479020.1; 105, KC479019.1; Muzaffargarh3, MH608290.1; Kasur2, MH608289.1; Muzaffargarh2, MH549199.1; Muzaffargarh1, MH549198.1; Rawalpindi2, MH549197.1; Rawalpindi1, MH549196.1; Kasur1, MH549195.1; 46, KT357370.1; 37y97, KT357369.1; Ranana, EU118961; 611, EF636663; Nether, KC935387.1; NGR, JN982341.1; TWN1, EF551366.1; TWN3, EF651794.1; TWN17, HQ009756.1; TWN4, EU139491.1; TWN2, EF560599.1; DB63, MG905720.1; B2-15, MG905719.1; B1-17, MG905718.1; B1-7, MG905717.1; B16, MG905716.1; B12, MG905714.1; DB51, MG905713.1; mkk2, MG905711.1; XJ-2, MN366176; XJ-6, MN366177; XJ-21, MN366178; XJ-35, MN366179.