Figure S5.

Data Collection and Processing Pipeline for the Cryo-EM Structure of the C105-SARS-CoV-2 S Complex, Related to Figure 5

(A) Representative micrograph of C105-S complex in vitreous ice. Inset: Power spectrum of micrograph determined during CTF estimation showing Thon rings to 3.2 Å. (B) Reference-free 2D classification of extracted particles. (C) Workflow for classification and refinement of selected particles. Briefly, after selection of good 2D class averages, an ab initio model was generated, which was then homogeneously refined before further 3D classification. To improve features at the SARS-CoV-2 RBD-C105 Fab interface, particles from states 1 and 2 were combined and used for non-uniform, focused refinement to yield a state 1-like reconstruction to an FSC = 0.143 resolution of 3.4 Å.