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. 2020 May 28;21(11):3851. doi: 10.3390/ijms21113851

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Br-EVs are enriched in interacting partners of the clathrin-independent carrier/GPI-AP-enriched compartments (CLIC/GEEC) cargo. (A) Heatmap visualization of quantitative proteomics analyses demonstrating the significantly differentially expressed proteins (p ≤ 0.05) in Br-EVs vs. P-EVs (red demonstrates upregulation in Br-EVs). (B) Functional enrichment analysis of proteins upregulated in P-EVs (blue) and Br-EVs (orange). (C) Quantification of surface localization of membrane-associated proteins upregulated in Br-EVs, CD63 serves as positive control (mean ± SD; three independent experiments). Statistical analysis was performed using unpaired two-tailed Student’s t-test (* p ≤ 0.05).