Table 3.
Characteristics of molecular products and number/share of plants with polymorphisms detected with RAPD, ISSR, and SCoT markers.
| Primer Symbol |
Primer Sequence 5′→3′ |
Marker Size [bp] |
No. of Markers Per Sample | Total Poly. Markers [%] |
No. and (%) of Plants with Polymorphism | ||||
|---|---|---|---|---|---|---|---|---|---|
| Min. | Max. | Total | Mono. | Poly. | Spec. | ||||
| RAPD | |||||||||
| R-A | GAC CGC TTG T | 655 | 2954 | 5 | 5 | 0 | 0 | 0.0 | 0 (0.0) |
| R-B | GGA CTG GAG T | 308 | 1505 | 6 | 6 | 0 | 0 | 0.0 | 0 (0.0) |
| R-C | GCT GCC TCA GG | 500 | 1840 | 8 | 8 | 0 | 0 | 0.0 | 0 (0.0) |
| R-D | CAA TCG CCG T | 36 | 1069 | 6 | 6 | 0 | 0 | 0.0 | 0 (0.0) |
| R-E | GGT GAC GCA G | 151 | 1004 | 9 | 9 | 0 | 0 | 0.0 | 0 (0.0) |
| R-F | CCC AGT CAC T | 96 | 1005 | 9 | 7 | 1 | 1 | 22.2 | 1 (1.2) |
| ∑ | 43 (7.2) |
41 (6.8) |
1 (0.2) |
1 (0.2) |
(3.7) | 1 (1.2) |
|||
| (mean from a single primer) | |||||||||
| ISSR | |||||||||
| I-A | GAG GGT (GGA)2 TCT | 200 | 2063 | 8 | 3 | 5 | 0 | 62.5 | 2 (2.5) |
| I-B | C (GAGA)4 | 723 | 1426 | 5 | 4 | 0 | 1 | 20.0 | 1 (1.2) |
| I-C | C (AGAG)4 | - | - | - | - | - | - | - | - |
| I-D | (GACA)4 | 333 | 1366 | 7 | 5 | 2 | 0 | 28.6 | 2 (2.5) |
| I-E | (GA)9 T | - | - | - | - | - | - | - | - |
| I-F | GT (GAGA)4 | - | - | - | - | - | - | - | - |
| ∑ | 20 (6.7) |
12 (4.0) |
7 (2.3) |
1 (0.3) |
(37.0) | 3 (3.8) |
|||
| (mean from a single primer) | |||||||||
| SCoT 1 | |||||||||
| S-A | CAA CAA
TGG CTA CCA CCG |
420 | 850 | 5 | 5 | 0 | 0 | 0.0 | 0 (0.0) |
| S-B | CAA CAA TGG CTA CCA CCT |
310 | 3158 | 15 | 15 | 0 | 0 | 0.0 | 0 (0.0) |
| S-C | ACC ATG GCT ACC ACC GTC |
325 | 880 | 8 | 8 | 0 | 0 | 0.0 | 0 (0.0) |
| S-D | ACC ATG GCT ACC ACC GTG |
393 | 2290 | 8 | 8 | 0 | 0 | 0.0 | 0 (0.0) |
| S-E | CCA TGG CTA CCA CCG CCA |
375 | 1182 | 7 | 7 | 0 | 0 | 0.0 | 0 (0.0) |
| S-F | CCA TGG CTA CCA CCG CAG |
214 | 2320 | 8 | 8 | 0 | 0 | 0.0 | 0 (0.0) |
| ∑ | 51 (8.5) | 51 (8.5) | 0 (0.0) | 0 (0.0) | (0.0) | 0 (0.0) | |||
| (mean from a single primer) | |||||||||
1 RAPD, randomly amplified polymorphic DNA; ISSR, inter-simple sequence repeats; SCoT, start codon target polymorphism; mono., monomorphic; poly., polymorphic; spec., specific (unique; present in a single band profile); total poly., share of polymorphic and specific markers (together) in the total number of markers.