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Representative 15% polyacrylamide gel electrophoresis under native conditions of the oligonucleotide samples at 1 μM concentration (only NU was analyzed at 2 μM conc.) in the selected K+- (a) and Na+-rich (b) buffer solutions, run at 80 V at room temperature (r.t.) for 2 h in Tris-Borate-EDTA (TBE) 1X buffer; lane 1: NU; lane 2: NU172; lane 3: cycNU172-EG2; lane 4: cycNU172-EG3; lane 5: cycNU172-Ph; lane 6: cycNU172-Pro.
