Figure 3.

Quantification of the MSMB removal during in vitro capacitation (IVC). The baseline fluorescent intensity mean of ejaculated spermatozoa was defined as 100%, to which the other IVC sperm groups were compared. (A) The decrease in fluorescent intensity mean in IVC spermatozoa treatment groups, i.e., non-inhibited, proteasomally-inhibited, E1-inhibited, and vehicle control. (B) Graphic representation of fluorescent intensity means in all treatment groups. Results are presented as the mean ± SD of four independent biological replicates. Statistical significance (p < 0.05) is indicated by superscripts.