Figure 5.

Densitometric quantification of 12 kDa immunoreactive MSMB bands from Figure 4 in protein extracts of ejaculated spermatozoa and all capacitated sperm treatment groups. The relative density of MSMB in the blot was calculated as the ratio of the optical density of anti- MSMB and anti-α-tubulin antibodies; the MSMB amount in the ejaculated sperm sample was defined as 100%, and all IVC sperm treatment groups were compared to ejaculated spermatozoa. Results are presented as the mean ± SD of four independent biological replicates. Statistical significance (p < 0.05) is indicated by superscripts.