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. 2020 Jun 10;21(11):4151. doi: 10.3390/ijms21114151

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Immunodetection of porcine MSMB in (poly)ubiquitinated protein sample isolated from the extract of whole ejaculated and IVC spermatozoa using the Signal-Seeker^TM Ubiquitination Detection kit (Ubiq. proteins—Binding fraction) with control detection of polyubiquitinated proteins and reciprocal detection of ubiquitinated proteins in MSMB immunoprecipitate (IP MSMB) from the extract of ejaculated spermatozoa with control detection of MSMB. Black arrows show MSMB (12, 17 and 22 kDa); asterisk indicates antibody heavy chains.