Figure 3.

Mutation in the hol gene prevents the lysis of H. influenzae cells by HP1 phage in vivo. H. influenzae Rd30 cells, non-lysogenized (○) or lysogenized with wt HP1 phage (▲) or HP1Δhol phage (■) were growth until exponential phase and phage lytic cycle was induced by addition of mitomycin C (black arrow). The culture turbidity (OD₆₀₀) was checked every 30 min. Results are representative of three independent experiments.