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. 2020 Jun 4;21(11):4013. doi: 10.3390/ijms21114013

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Growth of E. coli cells carrying pMPMT4Ω with cloned HP1 lytic genes: holin (H), HP1 endolysin (E), or both (HE) with and without induction with arabinose. (A) Viable cells count (CFU/mL). (B) Culture turbidity OD600. Induction of lytic genes expression with arabinose took place after 120 min of growth (black arrow). H-cells carrying hol gene, induced (Hind) or not (Hni) with arabinose. E-cells carrying lys gene, induced (Eind) or not (Eni) with arabinose. HE-cells carrying both lytic genes lys and hol, induced (HEind) or not (HEni) with arabinose. C-control cells carrying empty plasmid, induced (Cind) or not (Cni) with arabinose. Results are representative of three independent experiments.