Figure 8.

Inhibition of the sec system and the phage HP1 release by sodium azide in lysogenic H. influenzae Rd30 (HP1) induced with mitomycin C. Cells were grown to an OD₆₀₀ of 0.4 and mitomycin C was then added (time 0). Then, 1 mM sodium azide was added once, at time 0 or 60 or 90 or 120 min after induction and the phage titer was then measured by the double layer agar method for plaque assay. (○) Sodium azide added together with mitomycin C, at time 0; (□) Sodium azide added 60 min after induction; (▲) Sodium azide added 90 min after induction; (■) Sodium azide added 120 min after induction; and (●) control culture, induced with mitomycin C but not treated with sodium azide. Results are representative of three independent experiments.