Table 1.
Primer sequences.
| Primer Name |
Sequence 5′-3′ | Used for |
|---|---|---|
| PLLE PLPH |
CGGGAATTCATGAGTAAAAAATTTGGTGCA GTCAAGCTTTTTTACTCATCTATTGCTCC |
Amplification of hol from HP1 genome (320 bp) |
| PLLS PLPS |
CATGAATTCACTGTGCTGACGTATAACAAT GTACTGCAGAATACAGCCTAAAAACACAAT |
Amplification of lys from HP1 genome (619 bp) |
| PLPH PLPS |
GTCAAGCTTTTTTACTCATCTATTGCTCC GTACTGCAGAATACAGCCTAAAAACACAAT |
Amplification of hol lys from HP1 genome (918 bp) |
| HNHolL HNHolR |
TGGCTAGCTGTATGAATAGCAAAATAGATAGCGCA CGCAAGCTTTTACTCATCTATTGCTATCCCTA |
Addition of HisTag at N-terminus of holin |
| HCHolL HCHolR |
GACCATGGATGAATAGCAAAATAGATAGCGCA GCTCGAGACACTCATCTATTGCTATCCCTA |
Addition of HisTag at C-terminus of holin |