Figure 10.

MIA affects the levels of SNARE complex components in the brain of offspring rat. Effects of MIA on synaptic proteins SNARE complex in the cerebral cortex of rat offspring. Offspring male rats at PND 53–54 were sacrificed and brain tissues were collected. Immunoreactivities of synaptobrevin (VAMP1/2) (a), syntaxin-1 (d) and SNAP25 (g) normalized to GAPDH were analyzed by SDS-PAGE and Western blot. The mRNA levels of Vamp1 (b) and Vamp2 (c) of Stx1a (e) and Stx1b (f), and Snap25 (h) were determined using real-time PCR and normalized to Actb (β-actin). Representative Western blots are presented. Results of densitometric analysis of the investigated proteins and gene expressions are presented as the mean value ± S.E.M. (a–n = 9–10, b–n = 3–5, c–n = 3–5, d–n = 6–7, e–n = 3–5, f–n = 3–5, g–n = 8–9, h–n = 3–5). Data were analyzed using Student’s t-test. * p < 0.05, compared to control group.