Figure 11.

MIA evokes decrease of PSD-95 in offspring rats. The effects of prenatal MIA exposure on post-synaptic protein 95 (PSD-95) were analyzed in cerebral cortex of PND 53–54 offspring rats. (a) Immunoreactivity of PSD-95 protein and GAPDH were analyzed by SDS-PAGE and Western blot. Representative Western blots are presented. Results of densitometric analysis of the investigated protein are presented as the mean value ± S.E.M. (b) The mRNA level of PSD-95 gene, Dlg4, was determined using real time PCR and normalized to Actb (β-actin). Data represent the mean value ± S.E.M. (a–n = 8, b–n = 3–5). Data were analyzed using Student’s t-test. * p < 0.05 as compared to control group.