Figure 12.

MIA reduces the level of synaptic scaffold proteins—SHANK. Effects of MIA on post-synaptic SHANK protein family members were analyzed in the cerebral cortex of PND 53–54 rats. Immunoreactivities of SHANK1 (a), SHANK2 (c), SHANK3 (e), and GAPDH were analyzed by SDS-PAGE and western blot with representative blots presented. The mRNA levels of Shank1-3 (b,d,f) were determined using real-time PCR normalized to Actb (β-actin). Data represent the mean value ± S.E.M. (a–n = 3, b–n = 5–6, c–n = 3–4, d–n = 6–5, e–n = 3–4, f–n = 4). * p < 0.05, ** p < 0.01 compared to control group using Student’s t-test.