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. 2020 May 28;21(11):3823. doi: 10.3390/ijms21113823

Figure 4.

Figure 4

Effect of ferrocene-1H-1,2,3-triazole hybrids on iNOS expression and NO production in RMCs. Cells were pretreated with the compounds at 12.5 μg/mL for 2 h and subsequently stimulated with TNF-α (10 ng/mL) for 24h. The expression of iNOS and NO in RMCs were determined by (a) real-time PCR analysis and (b) nitric oxide assay, respectively. (c) Cells were incubated without or with F, X1, X2, X3, X4, or X5 at 12.5 μg/mL for 24 h. The mRNA was extracted, reverse transcribed, and then analyzed the iNOS expression by real-time PCR. Data are expressed as fold of control (1% DMSO-treated group) and each column represents the mean ± SEM of at least three repeated and independent assays. Asterisks indicate a significant difference compared with the control group, ** p < 0.01. Hashes indicate a significant difference compared with the TNF-α-treated group, ## p < 0.01.