Gene expression analyses in the root tissue of tomato plants subjected to different nutritional regimes. The levels of expression of SlTOM1.1 (A), SlTOM1.2 (B), SlTOM1.3 (C) and SlYSL1 (D) were assessed by quantitative Real Time RT–PCR. The data were normalised to two internal controls: Tomato LeEF-1 mRNA for elongation factor 1 alpha and Tomato ubi3 gene for ubiquitin. The relative expression ratios were calculated using C plants as a calibrator sample. The values reported are means ± SE (n = 3). The statistical significance was tested by means Student′s t test (*, p < 0.05; **, p < 0.01; ***, p < 0.001).