Figure 3.

A15-1 aptamer detects M. hyorhinis but not other mycoplasma infections. (A) Relative abundance of mycoplasma species from the contaminated cells of other laboratories. MP^a, MP^b, and MP^c represent different species of mycoplasma from the three laboratories, respectively; (B) A15-1 (blue) binding test in other species of mycoplasma-infected Jeko-1 cells by flow cytometry. The control aptamer is L14-2 (red); (C) Fluorescence intensity measurement of A15-1 or L7-2 (control) aptamer-bound MP⁻ or MP⁺ Jeko-1 cells using a microplate reader. MP⁻, mycoplasma negative; MP^+(hyo), M. hyorhinis positive; MP^+(Unc), unclassified mycoplasma negative; MP^+(yea), mixed mycoplasma positive. Data show the mean ± SD of three independent experiments. “NS” means not significant.