Phylogenetic tree of OsCHSs and other plant CHS family members. The amino acid sequences were aligned with Clustal-W and the neighbor-joining tree was built with MEGA ver. 6. Amino acid sequences used were AtCHS (AAB35812), ZmCHS (NP_001142246.1) EaCHS (Q9MBB1), HvCHS (CAA41250), MsCHS2 (P30074), PnCHS (BAA87922), AmQNS (AGE44110), CsOLS (B1Q2B6), RpALS (AAS87170), PsSTS (CAA43165), WtPKS1 (AAW50921), MdBIS1 (NP_001315967), RdORS (BAV83003), AtPKSA (O23674), and AtPKSB (Q8LDM2). QNS, OLS, ALS, BIS, and ORS stand for quinolone synthase, olivetol synthase, aloesone synthase, 3,5-dihydroxybiphenol synthase, and orcinol synthase, respectively.