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CHS activity assay of recombinant OsCHS8 (a) and OsCHS24 (b). The tetrahydroxychalcone was formed from p-coumaroyl-CoA and malonyl-CoA by OsCHS8 and OsCHS24. The resulting chalcone was spontaneously converted to naringenin, which was analyzed by reversed-phase HPLC. (c) HPLC chromatogram of the authentic naringenin.
