Figure 5.

Characterization of the oschs24 mutant. (a) The gene structure of OsCHS24 showing the T-DNA insertion position of the oschs24 mutant. Black boxes, gray boxes, and lines between boxes indicate exons, UTRs and introns, respectively. A triangle represents the position of the T-DNA insertion in oschs24. (b) RT-PCR analysis of OsCHS24 expression in the leaves of homozygous oschs24 mutants. The homozygous oschs24 plants showed the suppressed expression of OsCHS24. OsUBQ5 was used as an internal control. (c) Accumulation of sakuranetin in wild-type and oschs24 mutant in response to UV irradiation. The leaf samples were collected from UV-treated rice plants 1, 2, and 4 days after UV irradiation. Analyses of the sakuranetin contents were performed on triplicate biological samples. The results represent the mean ± standard deviation. FW; fresh weight.