Table 2.
Metabolites putatively identified in roots and root exudates from potato cultivars differing in susceptibility to Spongospora subterranea f. sp. subterranea detected using UPLC-MS.
| Putative Identity | ESI * | m/z | Rt (min) | Roots | Root Exudates | Tolerant Inoculated | Tolerant Un-Inoculated | Susceptible Inoculated | Susceptible Un-Inoculated |
|---|---|---|---|---|---|---|---|---|---|
| Sugars | |||||||||
| D-mannitol | + | 181.0716 | 1.94 | + | + | ||||
| D-ribulose | − | 149.0454 | 2.34 | + | + | ||||
| Heterodendrin | + | 262.1291 | 2.57 | + | + | + | + | + | + |
| Linamarin | + | 248.1132 | 2.01 | + | + | ||||
| Nystose | − | 341.1081 | 2.33 | + | + | + | |||
| Sugar alcohol | |||||||||
| Lactitol dehydrate | − | 343.1237 | 2.15 | + | + | ||||
| Amino acids | |||||||||
| Arginine | − | 175.1197 | 1.85 | + | + | + | + | ||
| 4-Aminobutanoic acid | − | 102.0556 | 2.17 | + | + | ||||
| Glutamine | − | 145.0617 | 1.85 | + | + | ||||
| Kinetin | + | 136.0623 | 2.58 | + | + | ||||
| Leucine | − | 130.0870 | 2.66 | + | + | + | + | + | |
| Methionine | − | 150.0603 | 2.76 | + | + | ||||
| Methionine sulfoxide | − | 166.0543 | 4.40 | + | + | ||||
| 5-Oxoproline | − | 130.0506 | 3.95 | + | + | ||||
| Phenylalanine | − | 166.0846 | 4.77 | + | + | ||||
| Proline | + | 116.0711 | 2.15 | + | + | + | + | ||
| Pyrroline 5-carboxylate | + | 130.0504 | 1.84 | + | + | ||||
| Stachydrine | + | 144.1025 | 2.63 | + | + | + | |||
| Tryptophan | − | 203.0820 | 5.55 | + | + | + | + | ||
| Valine | − | 118.0869 | 2.82 | + | + | + | + | ||
| Fatty acids | |||||||||
| (6Z)-6-Decosenamide | + | 338.3443 | 6.22 | + | + | + | |||
| Linoleic acid amide | + | 280.2636 | 11.55 | + | + | + | |||
| Laestisaric acid | − | 295.2276 | 14.95 | + | + | ||||
| Octanoic acid | − | 145.1259 | 2.73 | + | + | ||||
| 16-Oxohexadecanoic acid | − | 269.2117 | 14.28 | + | + | ||||
| C16 sphinganine | + | 274.2741 | 10.41 | + | + | + | |||
| (11E)-9,10,13-Trihydroxy-11-Octadecenoic acid | + | 329.2329 | 10.98 | + | + | ||||
| Alkaloids | |||||||||
| Cyclopamine | + | 706.4505 | 8.09 | + | + | + | |||
| Dehydrosolasodine | + | 398.3254 | 9.93 | + | + | ||||
| Laurolitsine | + | 314.1389 | 8.91 | + | + | ||||
| Melicopicine | − | 328.1187 | 7.45 | + | + | + | |||
| Solanine | + | 722.4457 | 7.59 | + | + | ||||
| Solanidine | + | 398.3412 | 10.10 | + | + | + | + | + | |
| Solanidane | + | 383.6895 | 9.10 | + | + | ||||
| Solasodiene | + | 396.3255 | 10.31 | + | + | + | + | + | |
| Solasodine | + | 414.3361 | 8.53 | + | + | + | + | + | |
| Swainsonine | + | 174.1113 | 2.74 | + | + | ||||
| Trachelanthamidine | + | 142.1207 | 2.44 | + | + | + | + | ||
| Veratramine | + | 410.3049 | 9.00 | + | + | ||||
| Tomatidine | + | 416.3519 | 11.28 | + | + | + | |||
| Organic acids | |||||||||
| Azelaic acid | − | 187.0971 | 8.26 | + | + | + | + | ||
| Citric acid | − | 191.0194 | 2.58 | + | + | ||||
| Erythronic acid | + | 136.1032 | 2.72 | + | + | ||||
| Phenolics | |||||||||
| p-coumaric acid | + | 165.0557 | 2.91 | + | + | ||||
| 6-Hydroxymellein | + | 193.0501 | 7.63 | + | + | ||||
| Quinic Acid | − | 191.0536 | 2.22 | + | + |
+ The compound was present in abundance (high peak intensity) in the sample. Blank means that the compound was not abundant in the sample. * Electrospray ionization. m/z: mass-to-charge ratio Rt (min): Retention time in minutes.