Table 1.
Characteristics of Toll-like receptors (TLRs) of brown trout used in the present study. The chromosome number, location, transcriptional direction (F–forward and R–reverse), NCBI accession numbers, length of mRNA, and the amino acid sequences of each TLR are provided. The details of the predicted isoelectric point (pI), molecular weight (MW), and subcellular location are also shown.
| TLRs | Chromosome | Location | Orientation | mRNA Accession Number |
mRNA Length | Protein Accession Number |
Amino Acid Length |
pI | MW (kDa) | Subcellular Location |
|---|---|---|---|---|---|---|---|---|---|---|
| TLR1 | 3 | 32933053–32935476 | R |
XM_029731478.1* (TLR6) |
3149 |
XP_029587338.1* (TLR6) |
807 | 6.23 | 90.8 | Plasma membrane |
| TLR19 | 3 | 70531574–70546575 | F |
XM_029746142.1* (TLR12) |
5815 |
XP_029602002.1* (TLR12) |
972 | 6.15 | 109.9 | Plasma membrane |
| TLR13-like Chr1 | 1 | 76708863–76711946 | F | XM_029775870.1 | 4057 | XP_029631730.1 | 1027 | 6.01 | 117.4 | Membrane bound endoplasmic reticulum |
| TLR13-like Chr2 | 2 | 17203261–17205151 | R | XM_029691145.1 | 1422 | XP_029547005.1 | 322 | 6.73 | 38.4 | Plasma membrane |
| TLR13-like Chr6 | 6 | 49945705–49948596 | R | XM_029756725.1 | 3799 | XP_029612585.1 | 963 | 7.32 | 109.8 | Membrane bound mitochondria |
| TLR13-like Chr27 | 27 | 28493214–28496051 | F | XM_029717624.1 | 3451 | XP_029573484.1 | 945 | 6.42 | 108.4 | Membrane bound vacuoles |