Figure 5.

Cell cycle distribution of MCF-7-EV and HCT116-EV cells. Cells were untreated (control) or treated with IC₈₀ dosage for MCF-7 and IC₉₀ for HCT116 cells of C-1305 or C-1311 compounds for the times indicated and subjected to propidium iodide staining and flow cytometry as described in Materials and Methods. Histograms show number of cells (y-axis) versus DNA content (x-axis) and are representative of at least three experiments for each condition. Bar graphs show data quantitation with percentage of cells with less than 2N DNA (sub-G1 fraction).