Figure 6.

Cellular response of MCF-7-EV and HCT116-EV cells. (A) Representative pictures of changes in nuclear morphology of MCF-7 and HCT116 cells treated with IC₈₀ or IC₉₀ dosage, respectively, of C-1305 and C-1311 compounds. Cells were stained with 4′,6-diamidino-2-phenylindole (DAPI; 1 mg/mL) and visualized under fluorescent microscope (400× magnification); (B) Cytometric analysis of changes in mitochondrial transmembrane potential (ΔΨm) in MCF-7-EV and HCT116-EV cells treated with drugs for 120 h and labeled with JC-1 dye. Presented cytograms are representative of three independent experiments. Marked gates are populations of cells with decreased mitochondrial transmembrane potential (ΔΨm) (green fluorescence); (C) Phosphatidylserine externalization and membrane disruption in MCF-7-EV and HCT116-EV cells after 120 h of treatment with C-1305 and C-1311. Representative bivariate flow cytometry histograms of annexin V–fluorescein isothiocyanate (FITC) signal versus PI signal are shown. Bottom left quadrant represents live cells (annexin V negative, PI negative); bottom right quadrant represents early apoptotic cells (annexin V positive, PI negative); top right quadrant represents late apoptotic cells (annexin V positive, PI positive); top left quadrant represents primary necrotic cells (annexin V negative, PI positive).