Figure 2.

RNA-seq analysis of tricuspid valve (TV) transcriptomes indicate that Pergolide and Dexfenfluramine treatment shares the majority of differentially expressed genes (DEGs). (A): Principal component analysis (PCA) on tricuspid valve leaflets treated with water (green dots, n = 5), serotonin (orange dots, n = 5), pergolide (purple dots, n = 5), and dexfenfluramine (pink dots, n = 5). (B): Heatmap view of z-scored expression profile of DEGs in TV of rabbits treated with serotonin (n = 5), pergolide (n = 5), and dexfenfluramine (n = 5) compared to control (n = 5). (C): Venn diagram displays comparison between DEGs for each drug treatment. (D): Data of qRT-PCR (n = 5 per treatment) are reported as dot plots where each dot represents a rabbit and the horizontal bar indicates the mean ±SEM as compared to relative fold change obtained by RNA-seq analysis. # FDR <0.05, * p < 0.05 determined with one-way ANOVA followed by Dunnett’s multiple comparison test (n = 5 for each treatment). ^ <0.05 determined with Mann–Whitney test.