Figure 3.

Decreased metabolism and muscle structure are enriched by serotonin, pergolide, and dexfenfluramine treatment. AMPK activation by serotonin and pergolide. (A): Chord plot shows 46 common DEGs and their overlap between significant Gene Ontology (GO) terms. (B): Circular plot is colored according to z-score and highlights logFC of DEGs among significant GO terms. The outer circle shows a scatter plot for each term of the logFC of the assigned genes. Red and blue dots display individual up-regulated and down-regulated genes, respectively. The inner rectangles are sized to positively correlate with the significance of each GO term, and colored to represent the overall direction of change in expression of each individual term. (C): Western blotting detection of phosphorylation of AMPKα and total AMPKα in TV leaflet total protein extracts. Data are reported as dot plots where each dot represents a rabbit and the horizontal bar indicates the mean ±SEM. * p < 0.05, determined with one-way ANOVA followed by Dunnett’s multiple comparison test (n = 5 for each treatment).