Figure 12.

Diagram of experiment design in the present studyNeonatal hypoxia-ischemia was induced in 7-day-old (P7) Wistar rat pups. Sham-control and HI animals were subcutaneously injected with TSA (0.2 mg/kg body weight) or vehicle (10% DMSO in saline) at the same volume starting immediately after hypoxic exposure and lasting for 5 consecutive days (D 0–5). All animals dedicated to immunohistochemical studies received twice-daily intraperitoneal injections of BrdU (4–6 day after HI (D 4–6)). Animals in this group were perfused transcardially with 4% PFA 14 (D14) or 28 (D28) days after HI, and immunohistochemical analysis was performed. Western lot and PCR analysis were performed on non-perfused brains (1, 3 7 days after the insult (D 1–7)).