FIGURE 3.
The effects of maslinic acid on the phosphorylation level of ERK, CREB, PI3K and Akt in hippocampal tissue. Mice were treated with maslinic acid (1 or 3 mg·kg−1, p.o.), donepezil (DNZ; 5 mg·kg−1, p.o.) or the same volume of vehicle solution 1 hr before the kill. To examine the effect of scopolamine on the signalling molecules, scopolamine was treated 30 min before kill. The levels of p‐ERK/ERK, p‐CREB/CREB, p‐PI3K/PI3K and p‐Akt/Akt were measured in the naïve (a–d) and scopolamine‐induced memory impairment model (e–h). The immunoreactivity of each band was normalized to the control (taken as 1.0). Data were showed as means ± SEM (n = 5 per group; *P < .05, compared with the vehicle‐administered control group; # P < .05, compared with the scopolamine‐administered group; n.s., not significant)