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. 2020 May 29;21(11):3887. doi: 10.3390/ijms21113887

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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TRAIL sensitivity in LoVo and CD133^knockout LoVo cells (LoVo-CD133 KO cells). Three independent LoVo-CD133 KO cells (KO1, KO2, KO3) were generated using the CRISPR-Cas9 gene editing system and culturing a single colony. (A) Immunoblotting of CD133 expression in LoVo-CD133 KO cells. (B) Toxicity of TRAIL in LoVo-CD133 KO cells. TRAIL reduced the viability of LoVo cells, but TRAIL-dependent toxicity was not observed in all the KO cells. Data are presented as the mean ± SE of three independent experiments. (C) Sensitivity to TRAIL by LoVo and LoVo-CD133 KO1 cells. TRAIL-induced apoptosis was analyzed by Annexin-V/7-AAD staining and flow cytometry.