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. 2020 May 27;21(11):3815. doi: 10.3390/ijms21113815

Figure 6.

Figure 6

The response of distal and proximal parts of the flower AZ to the inhibition of polar auxin movement involved superoxide dismutase (SOD) activity. The SOD isoenzyme profile was obtained for the distal and proximal parts of the AZ. Tween 20 solution (0.05%) was applied directly to the inactive AZ (IN) and material was collected after 8 h. An inhibitor of polar auxin transport, namely 2,3,5-triiodobenzoic acid (TIBA, 0.05 mM in 0.05% Tween 20), was also applied to the IN AZ and tissues were excised 2, 4, 6, and 8 h after treatment. Mn-SOD (A) and two isoforms of Cu/Zn-SOD (B) were detected using in-gel assays (representative micrographs of nitroblue tetrazolium (NBT)-stained gel are presented). The charts below the pictures display the average densitometric data corresponding to the bands detected in three separate gels. Each band was quantified and expressed as the relative value of its intensity compared to the control AZ, which was considered to have a value of 1. Error bars indicate SEs. The values for each isoform were normalized to the control, namely the inactive AZ (IN). t-test: * p < 0.05, distal versus proximal part; a p < 0.05, treated distal part versus inactive distal part; b p < 0.05, treated proximal part versus inactive proximal part.