Figure 1.

Effects of cholesterol depletion on cold-induced responses of mTRPA1. (a and b) Representative traces of [Ca²⁺] change induced by cooling (upper panels) in the control condition (a) and after pretreatment with 10 mM MCD (b). Application of AITC (100 µM) was used as control for mTRPA1 activation. (c and d) Amplitudes of [Ca²⁺] responses evoked by cold (c) and AITC (d) in control condition (n = 118) or after pretreatment with 1 (n = 149), 5 (n = 202) or 10 mM (n =130) of MCD. (e and f) Maximal amplitudes of the first time derivative of the intracellular Ca²⁺ signal elicited by cold (e) and AITC (f) in control condition or after treatment with 1, 5 or 10 mM of MCD (the n numbers are the same as in panels c and d). The symbols *, ** and *** indicate p < 0.05, p < 0.01 and p < 0.001, respectively; Kolmogorov–Smirnov test.