Table 3.
Sequences of primers used.
| Gene | Forward Sequence (5′→3′) | Reverse Sequence (5′→3′) |
|---|---|---|
| ACTA2 | CCGGGAGAAAATGACTCAAA | GAAGGAATAGCCACGCTCAG |
| MYH11 | CAGATGCTGGACCTTGAAGA | TCCATGACCAGGATCTCATC |
| SM22 | CGCGAAGTGCAGTCCAAAATCG | GGGCTGGTTCTTCTTCAATGGGC |
| COL1A1 | AGCCAGCAGATCGAGAACAT | TCTTGTCCTTGGGGTTCTTG |
| TNC | CCAGCGACCATCAACGCAGC | GGGGCTTGTTCAGTGGATGCCT |
| FN1 | TCGAGGAGGAAATTCCAATG | ACACACGTCCACCTCATCAT |
| VCAN | GGGAACCTGGTGAAGAAACA | CTTCCACAGTGGGTGGTCTT |
| 18S rRNA | CGCCGCTAGAGGTGAAATTC | TTGGCAAATGCTTTCGCTC |
Abbreviations: ACTA2—actin alpha 2, MYH11—myosin-heavy chain 11, SM22—transgelin, COL1A1—collagen type I alpha 1 chain, TNC—tenascin C, FN1—fibronectin 1, VCAN—versican.