Figure 1.

Quality controls to check for contamination in seminal plasma supernatant. To check if the supernatant of the seminal plasma contains sperm cells, somatic cells, and genomic DNA, five seminal plasma samples were processed for RNA extraction and DNA extraction. MiR-191-5b and miR-10b-5p were measured by qPCR on cDNA transcribed for microRNAs. Messenger RNA of CD45, HPRT-1, and ACTB was measured by qPCR on cDNA transcribed for mRNAs. Markers of somatic DNA (HCK) and Y-chromosome-specific DNA (HY, DYS-1) were assessed on cDNA minus-RNA control samples. The genomic markers were also analyzed on DNA obtained from seminal plasma supernatant (“DNA from sup”) and seminal plasma pellet (“DNA from pellet”). Data are represented as means ± SD.