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. 2020 Jun 8;21(11):4089. doi: 10.3390/ijms21114089

Figure 2.

Figure 2

Optimization of microRNA acquirement from seminal plasma. MicroRNA yields were compared by qPCR between different RNA purification methods, with variation of the input volume of seminal plasma in the extraction procedure (25, 50, 100, 200 µL), with or without addition of an exosome enrichment kit, with or without incorporation of MS2 RNA carrier and proteinase K for the RNA extraction procedure, and with variation of the input volume of RNA in the cDNA reaction (1, 2, 4 µL). Data are represented as means ± SEM.