Table 1.
Primer sequences of mRNA- and genomic DNA targets 1.
| Forward Primer | Reverse Primer | |
|---|---|---|
| CD45 | 5′-GGTCGTCAAACAAAAACTTCCC-3′ | 5′-TGAGATCCATCCCTGCAGTG-3′ |
| HPRT-1 | 5′-AGATGGTCAAGGTCGCAAGC-3′ | 5′-TCAAGGGCATATCCTACAACAAAC-3′ |
| ACTB | 5′-ACCACACCTTCTACAATGAG-3′ | 5′-TAGCACAGCCTGGATAGC-3′ |
| HCK | 5′-TATTAGCACCATCCATAGGAGGCTT-3′ | 5′-CTTCCGCTCCACTTTCCCTAAC-3′ |
| HY | 5′-TGGCGATTAAGTCAAATTCGC-3′ | 5′-CCCCCTAGTACCCTGACAATGTATT-3′ |
| DYS-1 | 5′-TCCTGCTTATCCAAATTCACCAT-3′ | 5′-ACTTCCCTCTGACATTACCTGATAATTG-3′ |
1 Primers for CD45, hypoxanthine-guanine phosphoribosyltransferase-1 (HPRT-1), and beta-actin (ACTB) are directed against mRNA. Primers for HCK, HY, and DYS-1 are directed against genomic DNA.