Table 2.
MicroRNAs showing a significant difference between groups in the test phase 1.
| N vs. Low-C | N vs. Low-M | Low-C vs. Low-M | K-W | Average Cq | |
|---|---|---|---|---|---|
| miR-23b-3p | x | x | - | x | 28.6 |
| miR-27b-3p | x | x | - | x | 28.9 |
| miR-99b-5p | x | x | - | x | 29.8 |
| miR-152 | x | x | - | x | 30.8 |
| SNORD38b 2 | x | x | - | x | 33.7 |
| miR-204-5p | x | - | x | x | 31.7 |
| miR-221-3p | x | - | x | x | 32.1 |
| miR-34b-5p 2 | x | - | x | x | 32.3 |
| miR-146b-5p | x | - | x | x | 32.6 |
| miR-510 | x | - | x | x | 37.9 |
| miR-100-5p | - | x | x | x | 31.1 |
| miR-509-3p | - | x | x | x | 39.9 |
| miR-891a | x | - | - | - | 27.2 |
| miR-888-5p | x | - | - | - | 28.2 |
| miR-25-3p | x | - | - | - | 29.5 |
| miR-574-3p | x | - | - | x | 30.3 |
| miR-374b-5p | x | - | - | - | 30.3 |
| miR-30d-5p | x | - | - | - | 31.4 |
| miR-598 | x | - | - | - | 33.6 |
| miR-32-5p | x | - | - | x | 33.6 |
| miR-16-1-3p | x | - | - | x | 33.9 |
| miR-501-5p | x | - | - | - | 34.1 |
| miR-590-3p | x | - | - | - | 34.7 |
| miR-339-3p | x | - | - | x | 35.2 |
| miR-34c-3p | x | - | - | - | 36.7 |
| miR-99a-5p | - | x | - | - | 26.4 |
| miR-106a-5p | - | x | - | - | 27.8 |
| miR-24-3p | - | x | - | - | 28.4 |
| let-7d-3p | - | x | - | - | 31.6 |
| miR-376c-3p | - | x | - | - | 33.9 |
| miR-328 | - | x | - | - | 34.1 |
| miR-665 | - | x | - | x | 36.1 |
| miR-142-3p | - | x | - | - | 37.0 |
| miR-455-5p | - | x | - | - | 37.8 |
| miR-26a-5p | - | - | x | - | 27.1 |
| miR-20a-5p | - | - | x | - | 28.8 |
| miR-205-5p | - | - | x | - | 28.1 |
| miR-222-3p | - | - | x | - | 29.4 |
| miR-196b-5p | - | - | x | x | 30.5 |
| miR-532-5p | - | - | x | - | 32.1 |
| miR-892a | - | - | x | - | 32.1 |
| miR-18b-5p | - | - | x | - | 33.0 |
| miR-202-5p | - | - | x | x | 34.7 |
| miR-455-3p | - | - | x | - | 35.3 |
| miR-500a-5p | - | - | x | x | 35.3 |
| miR-502-3p | - | - | x | - | 35.8 |
| miR-31-3p | - | - | x | - | 35.9 |
| miR-181b-5p | - | - | x | x | 35.9 |
| miR-34b-3p | - | - | x | x | 36.2 |
| miR-202-3p | - | - | x | x | 36.8 |
| miR-502-5p | - | - | x | - | 37.4 |
| miR-449a | - | - | x | - | 37.9 |
| miR-335-3p | - | - | x | x | 38.9 |
| miR-509-3-5p | - | - | x | x | 39.2 |
1 MicroRNAs marked with x in the first three columns showed a significant difference (p ≤ 0.05) between two groups in a Mann–Whitney U test. MicroRNAs marked with x in the fourth column showed the most significant difference (p < 0.1) among all groups in a Kruskal–Wallis test. N, normozoospermia samples; OL, oligozoospermia samples; AS, asthenozoospermia samples. Average Cq values of the nine samples in the test set are shown. 2 For both SNORD38b and miR-34b-5p, all samples in the normozoospermia group of the test cohort were positive, whereas all samples in one or two of the other groups showed a signal below the threshold. For both, average Cq was calculated for only the samples showing a significant expression.