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. 2020 Apr 30;7(12):2000871. doi: 10.1002/advs.202000871

Figure 5.

Figure 5

The effects of mutations in UBE2N on MavC‐induced ubiquitination. A) Detailed views of the interactions between MavC and UBE2N in the ternary complex, similar to Figure 4A. B) The effects of mutations in UBE2N on MavC‐induced ubiquitination. MavC was added to reactions containing ubiquitin, UBE2N or UBE2NR6A/R7A, UBE2NK10A/R14A, and UBE2N∆N6‐14 (deletion mutant missing the residues from Arg6 to Arg14) mutants. The reactions were allowed to proceed for 8 or 30 min at 37 °C. Samples resolved by SDS‐PAGE and visualized by Coomassie staining were quantified using Image Studio. The ratios are from three independent experiments. Error bars indicate standard error of the mean (SEM). C,D) The effects of mutations in UBE2N on MavC‐induced ubiquitination. MavC was added to reactions containing ubiquitin, UBE2N or its mutants. The reactions were allowed to proceed for 8 or 30 min at 37 °C before SDS‐PAGE and detection by Coomassie staining.