Figure 1.

Phylogenetic analysis of protein sequences of the 52 OeuAQPs and the 79 OleurAQPs with Fraxinus excelsior, Arabidopsis thaliana and Populus trichocarpa hortologs. Deduced amino acid sequences were aligned using ClustalW, and the phylogenetic tree was constructed using the maximum parsimony method. Maximum parsimony analysis was conducted using the subtree-pruning-regrafting algorithm. The number next to the branches represents bootstrap values ≥50% based on 5000 resampling. The distance scale denotes the number of amino acid substitutions per site. The AQP sequences from O. europaea var. sylvestris, O. europaea cv. Picual, F. excelsior, A. thaliana and P. trichocarpa are preceded by the prefixes Oeu, Oleur, Fraex, At and Poptr, respectively. OeuAQPs are mentioned in red, and OleurAQP in bold. The name of each subfamily and subgroup is indicated next to the corresponding group. XIP* and XIP** correspond to XIP sequences from Rosids and Asterids, respectively. OeuAQP accession numbers and sequences are listed in Figure 3 and Figure S1. OleurAQP and FraexAQP accession numbers and sequences are listed in Figures S2 and S3, respectively. This Figure 1 is also presented in Supplemental Figure S4 with high definition for ease of reading.