Figure 2.

(A) Phylogenetic relationship, (B) Exon/Intron genomic structure, and (C) protein motif organization of the 52 full-length Olea europaea var. sylvestris aquaporin sequences. (A) OleuAQP Sequences were aligned with MUSCLE, and the unrooted phylogenetic tree was reconstructed using the maximum likelihood method. The numbers at the nodes represent the percentage bootstrap values (only those >50% were represented). The reliability for the internal branch was assessed using 1000 bootstrap replicates. The distance scale denotes the number of amino acid substitutions per site. OleuAQPs clustered into five AQP subfamilies: OleuPIPs, OleuXIPs, OleuTIPs, OleuNIPS and OleuSIPs. (B) Exons and introns of the OleuAQP genes are represented by red boxes and black lines, respectively. Gene structures were compared using GSDS software. Gene orientations are indicated (5′—3′) in the x-axis. (C) Distribution of the conserved motifs among the OleuAQP proteins. Motif analysis was performed by using the MEME web server. Fifteen conserved motifs were identified, and the different motifs are identified using different colored boxes, as indicated at the bottom of the figure. Each color block in the different proteins indicates a specific motif, for which the amino acids are detailed in Figure S6. Protein orientations are indicated (Nter-Cter) on the x-axis.