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ID and radiation differentially up-regulate VEGF protein in MCF12A and MCF7 breast cells. MCF12A (A,B) and MCF7 (C,D) cells were cultured in iodine-containing medium for 7 days. Thereafter, the medium was replaced by iodine-containing (control) or iodine deficient (ID) medium and cells were irradiated with a dose of 0.1 (A, C) or 3 Gy (B, D). Cells were harvested 5 (A, B) or 7 (C, D) hours after medium change. VEGF protein expression was visualized by western blot. N = 3.
